Labuan Bajo Port has no single published opening-hours notice: it operates as a working harbour with activity from early morning (most Komodo trips leave 6:00–8:00 AM) into the evening for ferry and fishing traffic, but there is no confirmed 24-hour guarantee or dedicated left-luggage counter — bags are typically left aboard your booked boat or held by your hotel instead.
Two of the most common questions we see about this harbour are simple ones with no single official answer: what time does it open, and where do you leave your bags? Here is what we can say honestly, based on how tour operators and the ASDP ferry actually run, rather than inventing a schedule that does not exist.